Animal Feed Science and Technology

Effect of deep-stacking broiler litter on pathogenic bacteria, intake, digestibility, microbial protein supply and rumen parameters in sheep

a b s t r a c t

To assess the effectiveness of the deep-stacking process on pathogenic bacteria in broiler litter (BL), the raw BL was deep-stacked in 1 × 1 × 1.5 m plastic bins with 250 or 350 g mois- ture/kg atthe depths of 30, 60 and 120 cm for a period of 21 days. The chemical composition and microbial populations in raw and deep-stacked BL (DBL) were determined. The effect of feeding DBL on nutrient intakes, digestibility, microbial protein supply (MPS) and rumen parameters was assessed using different levels (0, 70, 140 or 210 g/kg diet dry matter [DM]) of DBL with 350 g moisture/kg in the diet of male Moghani sheep (four sheep per treatment), in a 28-day experiment. The deep stacking process of BL increased the contents of ash and ash-free neutral detergent fibre (NDFom), and decreased the crude protein (CP) and non- fibre carbohydrates (NFC) (P<0.05). Increasing moisture content resulted in increase of ash and reduction of NFC concentration in DBL (P<0.001). Compared to the raw BL, the popula- tions of total bacteria, Escherichia coli, and other Coliforms in DBL were decreased (P<0.05), with the exception of depth 120 cm at 250 g moisture/kg. No Salmonella population was observed at depths 30 and 60 cm with 250 and 350 g moistures/kg, and at depth 120 cm with 350 g moisture/kg (P<0.05). Dietary inclusion of DBL resulted in linear increase of the DM, organic matter (OM) and CP intakes and quadratic increases ofthe NDFom and ash-free acid detergent fibre intakes (P<0.05). The digestibility coefficients of DM, NDFom and CP were increased linearly (P<0.05), and OM digestibility increased quadratically (P=0.036) as the dietary level of DBL was raised. The MPS increased quadratically (P=0.011) with dietary inclusion of DBL. Adding DBL to the diet linearly decreased (P<0.05) the ruminal ammonia- N concentration and acetate to propionate ratio, and linearly increased (P<0.05) the total volatile fatty acids. The counts oftotal protozoa and sub-family Entoniniinae were decreased linearly (P<0.05) as the level of DBL in the diet was raised. Overall, to obtain a safe feedstuff, BL should be deep stacked at 350 g moisture/kg, as this led to a decrease in pathogenic bacteria numbers to a safe level for ruminant consumption. Compared to the control group, feeding sheep with DBL containing 350 g moisture/kg, up to 210 g/kg diet DM, improved feed intake, digestibility, MPS and ruminal fermentation, without any adverse effects on animal health. However, the maximum animal response was observed in the sheep fed 140 g DBL/kg diet DM.